Do this consistently on the same end of the slide to help orient your slide.īe patient and take the time to let your slide air dry before proceeding with adhering it to the slide. Be sure to label the far edge of the slide. It is very easy to get confused which side of the slide your smear is on. You have lots of room on your slide use it! It helps to initially draw a circle on the bottom of the slide so you know where to look for your smear. You are striving for a light suspension of cells that will leave a faint cloudy deposit on your slide. Dispose of your completed slides in the disinfectant bucket at your bench. Heat or methanol fixation is not guaranteed to kill the organism. The loop is very flexible and it is easy to zing off a loop-full of organisms. Materialsīe careful of aerosols when transferring bacteria from your loop to the slide. It will undoubtedly take you several tries before you are successful. While the goals are the same for both, evenly and lightly dispersed cells firmly adhered to the slide surface, the techniques are slightly different. You will be preparing slides for staining from both broth and agar surfaces. The cells typically shrink in size and will exhibit some changes in shape and extra-cellular matrices. All procedures that attach the bacteria to the slide result in some morphological changes. The bacteria need to be firmly attached to the slide so they are not washed off during the staining procedures.Large blobs of cells also do not stain properly and could yield erroneous results from the improper staining. If there are too many bacteria on the slide they will form a big glob and you will not be able to see the morphology of the individual cells.
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